Cytometric assessment of DNA damage induced by DNA topoisomerase inhibitors.

@article{Darzynkiewicz2009CytometricAO,
  title={Cytometric assessment of DNA damage induced by DNA topoisomerase inhibitors.},
  author={Zbigniew Darzynkiewicz and Dorota H Halicka and Toshiki P. Tanaka},
  journal={Methods in molecular biology},
  year={2009},
  volume={582},
  pages={
          145-53
        }
}
Exposure of cells to inhibitors of DNA topoisomerase I (topo I) or topoisomerase II (topo II) leads to DNA damage that often involves formation of DNA double-strand breaks (DSBs). DNA damage, particularly induction of DSBs, manifests by phosphorylation of histone H2AX on Ser-139 which is mediated by one of the protein kinases of the phosphoinositide kinase family, namely ATM, ATR, and/or DNA-PK. The presence of Ser-139 phosphorylated H2AX (gammaH2AX) is thus a reporter of DNA damage. This… CONTINUE READING

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