Cytolytic T cell hybridomas. I. Interleukin 2-independent noncytolytic variants.

Fluorescein-specific cytolytic hybrids were obtained by fusion of an interleukin 2 (IL2)-dependent murine cytotoxic T lymphocyte clone with the thymoma BW5147. The hybrid cells grow and retain cytolytic activity in medium with recombinant IL2. In medium without IL2 rare variants can be selected which grow in the absence of exogenous IL2; these variants are… CONTINUE READING