Cytological localization of Dengue-2 antigens: an immunological study with ultrastructural correlation.

@article{Cardiff1973CytologicalLO,
  title={Cytological localization of Dengue-2 antigens: an immunological study with ultrastructural correlation.},
  author={Robert Cardiff and S B Russ and W E Brandt and Ph. K. Russell},
  journal={Infection and immunity},
  year={1973},
  volume={7 5},
  pages={809-16}
}
Immunofluorescence and electron microscopy were used to study the development and cytological distribution of dengue-2 virion and nonvirion antigens in monkey kidney cells. The type and combination of fixatives were found to affect the intensity of fluorescence. Paraformaldehyde fixation alone resulted in a low level of fluorescence, but an additional fixation step with ethanol or acetone resulted in maximum staining. No fluorescence was obtained after fixation with glutaraldehyde alone or in… CONTINUE READING
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