Cytological Demonstration of the Clonal Nature of Spleen Colonies Derived from Transplanted Mouse Marrow Cells

@article{Becker1963CytologicalDO,
  title={Cytological Demonstration of the Clonal Nature of Spleen Colonies Derived from Transplanted Mouse Marrow Cells},
  author={Andrew Becker and Ernest A. McCulloch and James E. Till},
  journal={Nature},
  year={1963},
  volume={197},
  pages={452-454}
}
Normal mouse blood-forming tissue contains a class of cells which, on being transplanted into heavily irradiated mice, can proliferate and form macroscopic colonies in the spleen. Often, within a given colony, the cells which are observed indicate that differentiation is occurring along three lines, into cells of the erythrocytic, granulocytic and megakaryocytic series, respectively. Unique radiation-induced marker chromosomes were used to obtain direct cytological evidence that the spleen… 
Spleen Colonies in Mice: Karyotypic Evidence of Multiple Colonies from Single Cells
TLDR
Evidence is offered that a single cell from the donor may give rise to two, three or four colonies in the irradiated recipient, suggesting that each colony could be a clone of cells derived from a single progenitor cell in the inoculum.
Character of Primary and Secondary Colonies of Haematopoiesis in the Spleen of Irradiated Mice
TLDR
It was found that the haematopoietic cells in the colonies were most probably clones, and when cells from primary colonies were injected, the secondary colonies were smaller and contained fewer cells of the proerythroblast type.
Clonal nature of murine hemopoietic blast cell colonies
TLDR
It is suggested that murine hemopoietic blast cell colonies are clonal, similar to colony forming units (CFU)-GEMM.
THE DISTRIBUTION OF COLONY-FORMING CELLS AMONG SPLEEN COLONIES.
Progenitor cells that are recognized by their ability to form colonies of descendants in the spleens of irradiated mice have the capacity for self-renewal. The distribution of new colony-forming
A cytological study of the capacity for differentiation of normal hemopoietic colony‐forming cells
TLDR
Support is provided for the view that colony forming hemopoietic stem cells are multipotent, and that differentiation along more than one pathway can occur during the formation of macroscopic splenic colonies.
Studies on the haemopoietic stem cells of mouse foetal liver
TLDR
The postulate of classical haematological theories that a proportion of the cells in blood-forming organs is capable of indefinite division with renewal of the stem line and of differentiation into mature blood cells has thus been experimentally demonstrated.
Haemopoietic progenitor cells in peripheral blood
TLDR
From early experiments by Brecher and Cronkite on radioprotection in parabiotic animals it has become clear that HSC circulate in the blood stream, and observations were later extended to show that such CFU-s exist in the peripheral blood of rodents in a concentration of approximately one-hundredth of that in bone marrow.
Quantitative Histology of Spleen Colonies in Irradiated Mice
TLDR
It was found that the large majority of clones consisted of cells of either erythroid or myeloid or mega‐karyocytic type, and mixed clones were mixed with association of the various cell types in all possible combinations.
DECLINE IN COLONY-FORMING ABILITY OF MARROW CELLS SUBJECTED TO SERIAL TRANSPLANTATION INTO IRRADIATED MICE.
TLDR
It was found that the radiation sensitivity of colony-forming ability increases in cells subjected to serial transplantation, which suggests that the self-renewal process does not necessarily result in an exact replication of the original colony-formation cells.
Radiobiological studies on cultured mouse bone marrow cells in vitro.
TLDR
These cultured bone marrow cells showed neither protective ability to lethally irradiated mice nor colony forming ability in the spleen of recipient mice, when injected into 700 R-irradiated NC-mice.
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