Cytological Demonstration of the Clonal Nature of Spleen Colonies Derived from Transplanted Mouse Marrow Cells

@article{Becker1963CytologicalDO,
  title={Cytological Demonstration of the Clonal Nature of Spleen Colonies Derived from Transplanted Mouse Marrow Cells},
  author={A. Becker and E. McCulloch and J. Till},
  journal={Nature},
  year={1963},
  volume={197},
  pages={452-454}
}
Normal mouse blood-forming tissue contains a class of cells which, on being transplanted into heavily irradiated mice, can proliferate and form macroscopic colonies in the spleen. Often, within a given colony, the cells which are observed indicate that differentiation is occurring along three lines, into cells of the erythrocytic, granulocytic and megakaryocytic series, respectively. Unique radiation-induced marker chromosomes were used to obtain direct cytological evidence that the spleen… Expand
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Evidence is offered that a single cell from the donor may give rise to two, three or four colonies in the irradiated recipient, suggesting that each colony could be a clone of cells derived from a single progenitor cell in the inoculum. Expand
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DECLINE IN COLONY-FORMING ABILITY OF MARROW CELLS SUBJECTED TO SERIAL TRANSPLANTATION INTO IRRADIATED MICE.
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Radiobiological studies on cultured mouse bone marrow cells in vitro.
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These cultured bone marrow cells showed neither protective ability to lethally irradiated mice nor colony forming ability in the spleen of recipient mice, when injected into 700 R-irradiated NC-mice. Expand
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