Cytokinins in Azotobacter vinelandii Culture Medium.

  title={Cytokinins in Azotobacter vinelandii Culture Medium.},
  author={B J Taller and Tracy Yuk-Nar Wong},
  journal={Applied and environmental microbiology},
  volume={55 1},
Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentatively identified as trans-zeatin, isopentenyladenosine, and isopentenyladenine. The total cytokinin activity was equivalent to 0.75 mug of kinetin per liter.