Cytokine response of bovine mammary gland epithelial cells to Escherichia coli, coliform culture filtrate, or lipopolysaccharide.

@article{McClenahan2005CytokineRO,
  title={Cytokine response of bovine mammary gland epithelial cells to Escherichia coli, coliform culture filtrate, or lipopolysaccharide.},
  author={David J McClenahan and Josh P Sotos and Charles J. Czuprynski},
  journal={American journal of veterinary research},
  year={2005},
  volume={66 9},
  pages={1590-7}
}
OBJECTIVE To define the cytokine response of a cultured mammary gland epithelial cell line (ie, Mac-T) when incubated with Escherichia coli or its products. SAMPLE POPULATION Mac-T cells and E coli from cows with mastitis. PROCEDURE Mac-T cells were incubated with E coli or its products. The cytokine response of Mac-T cells to these treatments was quantified by measuring mRNA content of interleukin (IL)-1alpha, IL-1beta, IL-8, and tumor necrosis factor (TNF)-alpha by use of a quantitative… CONTINUE READING

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