Cytokine requirement for the development of T-lymphoid lineage potential in clonal lymphohaematopoietic progenitors in vitro.

@article{Ma2000CytokineRF,
  title={Cytokine requirement for the development of T-lymphoid lineage potential in clonal lymphohaematopoietic progenitors in vitro.},
  author={Feng Ma and Fan Chiang Yang and Akihisa Kaneko and Atsushi Manabe and Ryuhei Tanaka and Shigetaka Asano and Tatsutoshi Nakahata and Kohichiro Tsuji},
  journal={British journal of haematology},
  year={2000},
  volume={111 4},
  pages={1170-9}
}
The early process of T-cell development prior to thymic colonization has been poorly investigated because of the lack of a sensitive assay. We have developed a two-step in vitro culture system by combining a clonal culture with a fetal thymus organ culture (FTOC) and analysed the early development of T cells from lymphohaematopoietic progenitors. Cells of immature colonies derived from bone marrow cells of 5-fluorouracil (5FU)-treated mice using various combinations of early acting cytokines… CONTINUE READING