Cytokine production in NOD mice on prophylactic insulin therapy.

  title={Cytokine production in NOD mice on prophylactic insulin therapy.},
  author={E Strandell and Anne Kaas and Marie-Louise Hartoft-Nielsen and Troels G Bock and Karsten Buschard and Klaus Bendtzen},
  journal={APMIS : acta pathologica, microbiologica, et immunologica Scandinavica},
  volume={107 4},
We investigated whether cytokines produced primarily by monocytes/macrophages (IL-1alpha), Th1-lymphocytes (IFNgamma), or Th2-lymphocytes (IL-4) are modulated in diabetes-prone NOD mice by insulin treatment as used in prophylaxis studies. The cytokines were measured by ELISA in plasma and in supernatants of spleen cells activated ex vivo by lipopolysaccharide plus phytohemagglutinin. Insulin, 0.25-0.50 IU/day, was given subcutaneously for 8 weeks starting in 4-week-old female mice. The insulin… CONTINUE READING