Cytokeratin-like proteins in the sheep oocyte.

  title={Cytokeratin-like proteins in the sheep oocyte.},
  author={Laurence Gall and Patrick Le Guen and Daniel Huneau},
  journal={Cell differentiation and development : the official journal of the International Society of Developmental Biologists},
  volume={28 2},
In immunoblotting analysis of fully grown oocyte proteins separated by sodium dodecyl sulfate polyacrylamide (SDS-PAGE) gel electrophoresis, four polypeptides reacted specifically with cytokeratin antibodies: Mr 66,000, IEP 5.6; Mr 64,000, IEP 5.4; Mr 59,000, IEP 5.3; Mr 55,00, IEP 5.2. These proteins remained insoluble after extraction in high salt buffer and Triton X-100. In oocytes isolated from small antral follicles, only two polypeptides of Mr 66,000 and Mr 55,000 were detected… CONTINUE READING

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