Cytogenetic markers reveal a reinforcement of variation in the tension zone between chromosome races in the brachypterous grasshopper Podisma sapporensis Shir. on Hokkaido Island

  title={Cytogenetic markers reveal a reinforcement of variation in the tension zone between chromosome races in the brachypterous grasshopper Podisma sapporensis Shir. on Hokkaido Island},
  author={Beata Grzywacz and Haruki Tatsuta and Alexander G Bugrov and Elżbieta Warchałowska-Śliwa},
  journal={Scientific Reports},
The cytogenetic characteristics of the grasshopper Podisma sapporensis (two races 2n = 23♂ X0/XX and 2n = 22♂ neo-XY/neo-XX) were analysed through fluorescence in situ hybridization with rDNA and telomeric DNA probes, C-banding, fluorochrome and silver staining. For the first time, samples from the neighbourhood of a hybrid population (i.e., Mikuni Pass population) were studied. Our results indicated a significant degree of chromosomal differentiation between P. sapporensis races when comparing… 
Experimental hybridization between chromosome races of Podisma sapporensis Shiraki 1910 (Orthoptera, Acrididae) from Sakhalin and Kunashir Islands
Analysis of hybrid embryos in the crossing Sakhalin (female) x Kunashir (male) (C x K) revealed all theoretically expected genotypes of the offspring, and Cytogenetic analysis of the testes of the first generation of F1 hybrids did not reveal any disturbances in the meiosis.
Rapid chromosomal evolution in the bush-cricket Gonatoxia helleri Hemp, 2016 (Orthoptera, Phaneropterinae).
It is suggested that chromosomal translocations lead to diversification and speciation in this species and could be the driving force of adaptive radiation.
Evolution of chromosome number in grasshoppers (Orthoptera: Caelifera: Acrididae)
This study supports the view that chromosome numbers are much more stable across the investigated Caelifera compared to Ensifera, the second suborder of Orthoptera that includes the long antennae bush crickets and crickets.
Chromosomal evolution in the bush-cricket Gonatoxia helleri
  • 2020


Chromosome polymorphism and C-banding variation of the brachypterous grasshopper Podisma sapporensis Shir. (Orthoptera, Acrididae) in Hokkaido, northern Japan.
The considerable occurrence of polymorphism in chromosomes suggests that karyotypic diversification is undergoing in P. sapporensis and could provide a new perspective on the evolutionary process of different karyotype in Orthoptera.
Geographical patterns of chromosomal differentiation in the brachypterous grasshopper Podisma sapporensis (Orthoptera: Acrididae)
The analysis of the distribution of chromosomal races and subraces of P. sapporensis allows a reconstruction of the history of this species in the Okhotsk sea region and is proposed to divide it into two sibling species, which differ in the chromosome mechanisms of the sex determination system.
Relationship Between Chromosomal Races/Subraces in the Brachypterous Grasshopper Podisma sapporensis (Orthoptera: Acrididae) Inferred from Mitochondrial ND2 and COI Gene Sequences
The molecular phylogenetic tree revealed a high level of overall mitochondrial diversity, but the clustering of the examined population is in most cases closely related to their geographic distribution and associated with chromosomal races and subraces.
Different steps in the evolution of neo-sex chromosomes in two East African Spalacomimus species (Orthoptera: Tettigoniidae: Hetrodinae)
Variation in the evolution of two neo-XY sex chromosome systems are novel phylogenetic markers for the Hetrodinae and seem to promote speciation as important aspect for taxonomy.
A Comparative Chromosome Mapping Study in Japanese Podismini Grasshoppers (Orthoptera: Acrididae: Melanoplinae)
Karyotypes of 7 Japanese Podismini species are described and compared on the basis of conventional and molecular cytogenetic staining methods, and the monophyly of Tonkinacridina cannot be supported.
Molecular and classical chromosomal techniques reveal diversity in bushcricket genera of Barbitistini (Orthoptera).
The rDNA/NOR distribution and the composition of chromosome heterochromatin proved to be good cytogenetic markers for distinguishing species and phylogenetic lines and for understanding the genomic differentiation and evolution of Barbitistini.
Comparative analysis of chromosomes in the Palaearctic bush-crickets of tribe Pholidopterini (Orthoptera, Tettigoniinae)
A comparison of the location of the single r DNA/NOR in the genus Pholidoptera suggests that reduced chromosome number results from Robertsonian translocation between two pairs of autosomes, one carrying the rDNA/NOR.
B chromosomes, translocation between B and autosomes, and C-heterochromatin polymorphism of the grasshopper Podisma sapporensis Shir. (Orthoptera, Acrididae) in Hokkaido, northern Japan.
Seven categories of B chromosomes found in the brachypterus grasshopper Podisma sapporensis from Hokkaido populations differ in structure, size, and C-band content; an interaction between standard and non-standard chromosomal set provides an insight into the integration of supernumerary chromosome.
Experimental hybridization between X0 and XY chromosome races in the grasshopper Podisma sapporensis (Orthoptera: Acrididae). II. Cytological analysis of embryos and adults of F1 and F2 generations
The hypothesis that chromosomal differences play a key role in restricting gene flow between the X0 and XY races of the brachypterous grasshopper P. sap- porensis is not supported.
Chromosomal Localization of Ribosomal and Telomeric DNA Provides New Insights on the Evolution of Gomphocerinae Grasshoppers
Most remarkably, Chorthippus jacobsoni showed the presence of telomeric repeats in the pericentric regions of the 3 biarmed chromosome pairs originated by centric fusion, thus suggesting that these rearrangements were not of the Robertsonian type but true centic fusion with a probable generation of dicentric chromosomes.