Cytogenetic anchoring of radiation hybrid and virtual maps of sheep chromosome X and comparison of X chromosomes in sheep, cattle, and human

@article{Goldammer2009CytogeneticAO,
  title={Cytogenetic anchoring of radiation hybrid and virtual maps of sheep chromosome X and comparison of X chromosomes in sheep, cattle, and human},
  author={Tom Goldammer and Ronald Marco Brunner and Alexander Rebl and Chun Hua Wu and Ko Nomura and Tracy L Hadfield and Jill F. Maddox and Noelle E. Cockett},
  journal={Chromosome Research},
  year={2009},
  volume={17},
  pages={497-506}
}
A comprehensive physical map was generated for Ovis aries chromosome X (OARX) based on a cytogenomics approach. DNA probes were prepared from bacterial artificial chromosome (BAC) clones from the CHORI-243 sheep library and were assigned to G-banded metaphase spreads via fluorescence in-situ hybridization (FISH). A total of 22 BACs gave a single hybridization signal to the X chromosome and were assigned out of 32 tested. The positioned BACs contained 16 genes and a microsatellite marker which… CONTINUE READING
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