Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes

@article{Liao2009CytogeneticAO,
  title={Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes},
  author={Hongqing Liao and Shuoping Zhang and Dehua Cheng and Qi Ouyang and Ge Lin and Yifan Gu and Changfu Lu and Fei Gong and Guang-xiu Lu},
  journal={Journal of Assisted Reproduction and Genetics},
  year={2009},
  volume={26},
  pages={583-589}
}
By comparing the chromosomal constitution among the arrested cleavage-stage embryos, blastocysts and human embryonic stem cells (hESCs) which are all derived from monopronuclear (1PN) zygotes, it is aimed to determine whether chromosomally normal embryos can be reliably selected by blastocyst culture. After 1PN zygotes are sequentially cultured for 5 days, the blastocysts and arrested cleavage-stage embryos were analyzed by fluorescence in situ hybridization (FISH) with probes for chromosomes… CONTINUE READING
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