Cytofluorometric quantification of cell-surface antigens by indirect immunofluorescence using monoclonal antibodies.

@article{Poncelet1985CytofluorometricQO,
  title={Cytofluorometric quantification of cell-surface antigens by indirect immunofluorescence using monoclonal antibodies.},
  author={Philippe Poncelet and Pierre Carayon},
  journal={Journal of immunological methods},
  year={1985},
  volume={85 1},
  pages={65-74}
}
A method has been developed to determine the absolute number of binding sites on lymphoid cells by flow cytometric analysis of indirect immunofluorescence data after saturation the related monoclonal antibodies (mAbs). First, the cell lines RPMI 8402, MOLT-4, CEM and HSB-2 were studied to determine the number of p67, T cell-associated antigen molecules expressed on their membrane, as judged by the binding of radiolabeled T101 (CD5) mAb. Then saturating doses of unlabeled T101 followed by… CONTINUE READING