Cytochrome c oxidase: evidence for interaction of water molecules with cytochrome a.

@article{Sassaroli1989CytochromeCO,
  title={Cytochrome c oxidase: evidence for interaction of water molecules with cytochrome a.},
  author={Massimo Sassaroli and Yen Choon Ching and Siddharth Dasgupta and Denis L. Rousseau},
  journal={Biochemistry},
  year={1989},
  volume={28 8},
  pages={3128-32}
}
The resonance Raman spectra of cytochrome c oxidase in protonated buffer compared to that in deuterated buffer indicate that water molecules are near the heme of cytochrome a. Differences in widths of the heme line at 1610 cm-1, after short exposure to D2O, and, additionally, of the heme line at 1625 cm-1, after long exposure, can be accounted for by changes in resonance vibrational energy transfer between modes of cytochrome a2+ and the bending mode of water molecules in the heme pocket. On… CONTINUE READING