Cytochrome c depletion upon expression of Bcl-XS.

  title={Cytochrome c depletion upon expression of Bcl-XS.},
  author={Jordan S. Fridman and Joshua David Parsels and Alnawaz Rehemtulla and Jonathan Maybaum},
  journal={The Journal of biological chemistry},
  volume={276 6},
We have shown previously that Bcl-XS causes acute cell death in 3T3 cells without activating caspases (Fridman, J. S., Benedict, M. A., and Maybaum, J. (1999) Cancer Res. 59, 5999-6004). In this study, we determined that the explanation for lack of caspase activation is the cellular depletion of cytochrome c. Electron microscopy revealed gross structural changes in the mitochondria of Bcl-XS-expressing cells; however, cytochrome c was not detected in cytosolic fractions from these cells… CONTINUE READING