Cytochrome P450 of small intestinal epithelial cells. Immunochemical characterization of the increase in cytochrome P450 caused by phenobarbital.

@article{Bonkovsky1985CytochromePO,
  title={Cytochrome P450 of small intestinal epithelial cells. Immunochemical characterization of the increase in cytochrome P450 caused by phenobarbital.},
  author={Herbert L. Bonkovsky and Hans Peter Hauri and Ulrich Marti and Rodolfo Gasser and Urs A. Meyer},
  journal={Gastroenterology},
  year={1985},
  volume={88 2},
  pages={458-67}
}
We have studied total cytochrome P450 and the major form of cytochrome P450 increased by phenobarbital in small intestinal epithelial cells and livers of male Sprague-Dawley rats. Using an improved method for preparing microsomes from intestinal epithelial cells, we find that concentrations of total cytochrome P450 in intestinal cell microsomes are 10% of those in liver microsomes, and that this percentage is unchanged after phenobarbital treatment. In untreated rats, less than 5% of total… CONTINUE READING