• Corpus ID: 28389041

Cytochrome P-450 hPCN3, a novel cytochrome P-450 IIIA gene product that is differentially expressed in adult human liver. cDNA and deduced amino acid sequence and distinct specificities of cDNA-expressed hPCN1 and hPCN3 for the metabolism of steroid hormones and cyclosporine.

@article{Aoyama1989CytochromePH,
  title={Cytochrome P-450 hPCN3, a novel cytochrome P-450 IIIA gene product that is differentially expressed in adult human liver. cDNA and deduced amino acid sequence and distinct specificities of cDNA-expressed hPCN1 and hPCN3 for the metabolism of steroid hormones and cyclosporine.},
  author={Toshifumi Aoyama and Shigeru Yamano and David J. Waxman and D P Lapenson and Urs A. Meyer and Volker Fischer and Rachel F. Tyndale and Toshiya Inaba and Werner Kalow and H. V. Gelboin},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 18},
  pages={
          10388-95
        }
}
Immunoblotting analysis of human liver microsome preparations revealed that human cytochrome P-450 PCN1 (hPCN1, Mr approximately 52,000) was expressed in each of 40 individual specimens examined. In about 10-20% of the livers, an immunologically related protein having a lower electrophoretic mobility (Mr approximately 52,500) was also detected. A single liver was found that expressed only the lower mobility protein, designated hPCN3, and RNA isolated from this liver was used to construct a… 

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References

SHOWING 1-2 OF 2 REFERENCES

DNA Cloning (Glover, D

  • 1985

DNA 8 , 1 - 14 Omura , T . , and

  • 1989