Corpus ID: 19055218

Cytochrome P-450 difference spectra. Type II interactions in insecticide-resistant and -susceptible houseflies.

  title={Cytochrome P-450 difference spectra. Type II interactions in insecticide-resistant and -susceptible houseflies.},
  author={A. Kulkarni and R. Mailman and R. Baker and E. Hodgson},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  volume={2 3},
The relationship between chemical structure and the difference spectra obtained with microsomes from the abdomens of insecticide-susceptible and -resistant houseflies was examined. The presence of a "sterically accessible" nitrogen atom with a pair of nonbonded electrons or an accessible oxygen atom in certain configurations appears to be of primary importance in the formation of type II difference spectra. However, factors such as steric hindrance and electronic configuration determine the… Expand
Spectral interactions of insecticide synergists with microsomal cytochrome P-450 from insecticide-resistant and susceptible house flies
The cytochrome P-450 from susceptible house flies, although reported previously not to exhibit type I difference spectra with many xenobiotics, was found to elicit this spectral response with several methylenedioxyphenyl compounds. Expand
Steric factors in the inhibitory interaction of imidazoles with microsomal enzymes.
It appears that both binding and inhibition depend on the accessibility of the non-bonded electrons on the nitrogen atom at position — 3 of the ring. Expand
Effect of storage on the biochemistry of cytochrome P-450 from the housefly, Musca domestica☆
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Microsomal cytochrome P-450 from the housefly, Musca domestica: Assay and spectral characterization
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The study of type II optical difference spectra using several ligands revealed that the basic requirements for this perturbation are the same as in other animal species, namely, a sterically accessible sp2 or sp3 nitrogen atom. Expand
Substituted imidazoles as inhibitors of microsomal oxidation and insecticide synergists
Abstract The inhibitory activity of forty-seven 1-, 2-, and 4(5)-substituted imidazoles toward microsomal epoxidation, hydroxylation, and N -demethylation in enzyme preparations from rat liver andExpand
An unusual effect of N-octylamine on the cytochrome b5 spectrum of insect and mammalian microsomes.
Microsomes from rat and mouse liver, but not those from housefly abdomens, exhibit similar unusual spectral interactions with n-octylamine when supplemented with the soluble factor from the hornworm. Expand
Occurrence and characterization of microsomal cytochrome P-450 in several vertebrate and insect species.
Both quantitative and qualitative differences exist between the microsomal cytochrome P-450s of various animal species but differences in enzyme activity cannot be correlated with any single spectral characteristic. Expand
Visible Spectra of Type II Cytochrome P450-Drug Complexes: Evidence that “Incomplete” Heme Coordination Is Common
The visible spectrum of a ligand-bound cytochrome P450 is often used to determine the nature of the interaction between the ligand and the P450. One particularly characteristic form of spectra arisesExpand
Multiplicity of cytochrome P-450 in microsomal membranes from the housefly, Musca domestica.
The heterogeneity of cytochrome P-450 in abdominal microsomes from the CSMA, SBO, Fc, Rutgers and Baygon strains of the housefly was examined by three different methods, and evidence for cytochromes P- 450 of different spectral characteristics was provided. Expand