Cytochrome P-450 dependent metabolism of testosterone in rat skin.

  title={Cytochrome P-450 dependent metabolism of testosterone in rat skin.},
  author={Hasan Mukhtar and Mohammad Athar and David R. Bickers},
  journal={Biochemical and biophysical research communications},
  volume={145 2},
The incubation of microsomes of whole skin, dermis and epidermis with 14C testosterone in the presence of NADPH resulted in the formation of 6 beta-, 7 alpha- and 16 alpha-testosterone. Maximum enzyme activity occurred in epidermal microsomes followed by dermis and whole skin. Epidermal testosterone hydroxylase activity required NADPH and oxygen and was found to be inhibited by SKF 525A and metyrapone. Our data strongly suggest that testosterone is metabolized by the cytochrome P-450 dependent… CONTINUE READING