Cytochrome C oxidase III interacts with hepatitis B virus X protein in vivo by yeast two-hybrid system.

@article{Li2004CytochromeCO,
  title={Cytochrome C oxidase III interacts with hepatitis B virus X protein in vivo by yeast two-hybrid system.},
  author={Dan Li and Xiao-zhong Wang and Jie-ping Yu and Zhi-xin Chen and Yue-hong Huang and Qi-min Tao},
  journal={World journal of gastroenterology},
  year={2004},
  volume={10 19},
  pages={2805-8}
}
AIM To screen and identify the proteins which interact with hepatitis B virus (HBV) X protein in hepatocytes by yeast two-hybrid system and to explore the effects of X protein in the development of hepatocellular carcinoma (HCC). METHODS With HBV X gene amplified by polymerase chain reaction (PCR), HBV X bait plasmid, named pAS2-1-X, was constructed by yeast-two hybridization system3 and verified by auto-sequencing assay. pAS2-1-X was transformed into the yeast AH109, and X-BD fusion protein… CONTINUE READING

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