Cytochemical Localization of Catalase in Leaf Microbodies (peroxisomes)

@article{Frederick1969CytochemicalLO,
  title={Cytochemical Localization of Catalase in Leaf Microbodies (peroxisomes)},
  author={Sue Ellen Frederick and Eldon H. Newcomb},
  journal={The Journal of Cell Biology},
  year={1969},
  volume={43},
  pages={343 - 353}
}
Segments of mature tobacco leaves were fixed in glutaraldehyde, incubated in medium containing 3,3'-diaminobenzidine (DAB) and hydrogen peroxide, and postfixed in osmium tetroxide. Electron microscopic observation of treated tissues revealed pronounced deposition of a highly electron-opaque material in microbodies but not in other organelles. The coarsely granular reaction product is presumably osmium black formed by reaction of oxidized DAB with osmium tetroxide. Reaction of the microbodies… CONTINUE READING
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Cytochemical localization

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Peroxidase in healthy and dis- 28

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The widespread occurrence of plant cytosomes resembling animal micro35:357

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Functions of microbodies (peroxisomes)

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