Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells

@article{Gomzikova2017CytochalasinBM,
  title={Cytochalasin B-induced membrane vesicles convey angiogenic activity of parental cells},
  author={Marina Gomzikova and Margarita N. Zhuravleva and Regina R. Miftakhova and Svetlana S. Arkhipova and Vladimir G. Evtugin and Svetlana F. Khaiboullina and Andrey P. Kiyasov and Jenny L Persson and Nigel P. Mongan and Richard G. Pestell and Albert Anatolevich Rizvanov},
  journal={Oncotarget},
  year={2017},
  volume={8},
  pages={70496 - 70507}
}
Naturally occurring extracellular vesicles (EVs) play essential roles in intracellular communication and delivery of bioactive molecules. Therefore it has been suggested that EVs could be used for delivery of therapeutics. However, to date the therapeutic application of EVs has been limited by number of factors, including limited yield and full understanding of their biological activities. To address these issues, we analyzed the morphology, molecular composition, fusion capacity and biological… 
Evaluation of Cytochalasin B-Induced Membrane Vesicles Fusion Specificity with Target Cells
TLDR
It is found that there is no statistically significant preference in PC3 CIMVs fusion with target cells of the same type, and the greatest impact on C IMVs entry into target cells is by the heterophilic interaction of CIMV membrane receptors with the surface proteins of target cells.
Angiogenic Activity of Cytochalasin B-Induced Membrane Vesicles of Human Mesenchymal Stem Cells.
TLDR
It is believed that human CIMVs-MSCs have similar content, immunophenotype, and angiogenic activity to those of the parental MSCs and could be used for cell free therapy of degenerative diseases.
Angiogenic Activity of Cytochalasin B-Induced Membrane Vesicles of Human Mesenchymal Stem Cells
TLDR
Human CIMVs-MSCs have similar content, immunophenotype and angiogenic activity to those of the parental MSCs and are believed to be used for cell free therapy of degenerative diseases.
Cytochalasin B Treatment and Osmotic Pressure Enhance the Production of Extracellular Vesicles (EVs) with Improved Drug Loading Capacity
TLDR
An engineering strategy is presented that enables upscaled EV production with increased loading capacity through the secretion of EVs from cells via cytochalasin-B (CB) treatment and reduction of EV intravesicular contents through hypo-osmotic stimulation.
Analysis of the Interaction of Human Neuroblastoma Cell-Derived Cytochalasin B Induced Membrane Vesicles with Mesenchymal Stem Cells Using Imaging Flow Cytometry
TLDR
The internalization of SH-SY5Y cell-derived CIMVs within MSCs and the ability of the CIMV to modulate immunophenotype of the recipient cells were shown, and further studies are required to determine the effect of C IMVs on pro- or antioncogenic phenotype and function of MSCS.
Cytochalasin-B-Inducible Nanovesicle Mimics of Natural Extracellular Vesicles That Are Capable of Nucleic Acid Transfer
TLDR
Cytochalasin-B-inducible nanovesicles exhibited the highest level of oligonucleotide accumulation in HEK293 cells and a loading capacity of 0.44 ± 0.05 pmol/µg.
Immunosuppressive properties of cytochalasin B-induced membrane vesicles of mesenchymal stem cells: comparing with extracellular vesicles derived from mesenchymal stem cells
TLDR
It is demonstrated that CIMVs-MSCs localized in liver, lung, brain, heart, spleen and kidneys 48 h after intravenous injection and can be detected 14 days after subcutaneous and intramuscular injection and supports their further preclinical testing as an effective therapeutic delivery modality.
Mesenchymal Stem Cell Derived Biocompatible Membrane Vesicles Demonstrate Immunomodulatory Activity Inhibiting Activation and proliferation of Human Mononuclear Cells
TLDR
The data suggest that CIMVs-MSCs have immunosuppressive properties, are potential agents for immunomodulating treatment, and are worthy of further investigation.
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