Cytochalasin B binding to rabbit proximal tubular basolateral membranes.

  title={Cytochalasin B binding to rabbit proximal tubular basolateral membranes.},
  author={Paul T Cheung and Marc R. Hammerman},
  journal={Kidney international},
  volume={35 6},
Cytochalasin B binds to the Na+-independent D-glucose transporter in non-renal tissues. We have shown previously that the Na+-independent D-glucose transporter of the rabbit renal proximal tubular cell is localized exclusively in the basolateral membrane. To determine whether cytochalasin B binds to this renal transporter we measured binding of [3H]cytochalasin B to proximal tubular basolateral membranes isolated from rabbit kidneys. A steady state of binding is reached by 15 minutes at 20… CONTINUE READING