Cytochalasin B and trichostatin a treatment postactivation improves in vitro development of porcine somatic cell nuclear transfer embryos.

@article{Beebe2009CytochalasinBA,
  title={Cytochalasin B and trichostatin a treatment postactivation improves in vitro development of porcine somatic cell nuclear transfer embryos.},
  author={Luke F S Beebe and Stephen J McIlfatrick and Mark B. Nottle},
  journal={Cloning and stem cells},
  year={2009},
  volume={11 4},
  pages={477-82}
}
Somatic cell nuclear transfer (SCNT) is a useful technique for the production of transgenic pigs that can be used for biomedical research. However, the efficiency of SCNT in pigs is low. In this study, we examined the effect of two postactivation treatments, cytochalasin B (CB) and trichostatin A (TSA), on the in vitro development of porcine SCNT embryos. Treating porcine parthenotes with 7.5 microg/mL CB for 3 h after electrical activation was effective in preventing the extrusion of the… CONTINUE READING

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