Cysteine-specific PEGylation of rhG-CSF via selenylsulfide bond.

@article{Kunstelj2013CysteinespecificPO,
  title={Cysteine-specific PEGylation of rhG-CSF via selenylsulfide bond.},
  author={Men{\vc}i Kunstelj and Katarina Fidler and {\vS}pela {\vS}krajnar and Maja Kenig and Vanja Smilovi{\'c} and Mateja Kusterle and Simon Caserman and Irena Zore and Vladka Gaberc Porekar and Simona Jev{\vs}evar},
  journal={Bioconjugate chemistry},
  year={2013},
  volume={24 6},
  pages={889-96}
}
A new PEGylation reagent enabling selective modification of free thiol groups is described in this article. The reagent was synthesized by attaching linear polyethylene glycol (PEG) N-hydroxysuccinimide to selenocystamine. The reaction was very fast, resulting in over 95% conversion yield. The active group of this new PEG-Se reagent is a diselenide, reacting with thiols via thiol/diselenide exchange reaction. Recombinant human granulocyte colony-stimulating factor (rhG-CSF) with an unpaired… CONTINUE READING
1 Citations
0 References
Similar Papers

Citations

Publications citing this paper.

Similar Papers

Loading similar papers…