Cysteine-scanning analysis of putative helix XII in the YgfO xanthine permease: ILE-432 and ASN-430 are important.

@article{Papakostas2008CysteinescanningAO,
  title={Cysteine-scanning analysis of putative helix XII in the YgfO xanthine permease: ILE-432 and ASN-430 are important.},
  author={Konstantinos Papakostas and Ekaterini Georgopoulou and Stathis Frillingos},
  journal={The Journal of biological chemistry},
  year={2008},
  volume={283 20},
  pages={13666-78}
}
Transmembrane helix XII of UapA, the major fungal homolog of the nucleobase-ascorbate transporter (NAT/NCS2) family, has been proposed to contain an aromatic residue acting as a purine-selectivity filter, distinct from the binding site. To analyze the role of helix XII more systematically, we employed Cys-scanning mutagenesis of the Escherichia coli xanthine-specific homolog YgfO. Using a functional mutant devoid of Cys residues (C-less), each amino acid residue in sequence… CONTINUE READING

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