Cysteine Sulfoxidation Increases the Photostability of Red Fluorescent Proteins.

  title={Cysteine Sulfoxidation Increases the Photostability of Red Fluorescent Proteins.},
  author={Haiyan Ren and Bing Yang and Cheng Ma and Ying S. Hu and Peng George Wang and Lei Chuang Wang},
  journal={ACS chemical biology},
  volume={11 10},
Photobleaching of fluorescent proteins (FPs) is a major limitation to their use in advanced microscopy, and improving photostability remains highly challenging due to limited understanding of its molecular mechanism. Here we discovered a new mechanism to increase FP photostability. Cysteine oxidation, implicated in only photobleaching before, was found to drastically enhance FP photostability to the contrary. We generated a far-red FP mStable by introducing a cysteine proximal to the… CONTINUE READING
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