Cysteine Mutagenesis and Computer Modeling of the S6 Region of an Intermediate Conductance IKCa Channel

@article{Simes2002CysteineMA,
  title={Cysteine Mutagenesis and Computer Modeling of the S6 Region of an Intermediate Conductance IKCa Channel},
  author={Manuel Jos{\'e} Vieira Sim{\~o}es and L. Garneau and H{\'e}l{\`e}ne Hoblingre Klein and Umberto Banderali and Fadi Hobeila and Beno{\^i}t Roux and Lucie Parent and R{\'e}my Sauv{\'e}},
  journal={The Journal of General Physiology},
  year={2002},
  volume={120},
  pages={99 - 116}
}
Cysteine-scanning mutagenesis (SCAM) and computer-based modeling were used to investigate key structural features of the S6 transmembrane segment of the calcium-activated K(+) channel of intermediate conductance IKCa. Our SCAM results show that the interaction of [2-(trimethylammonium)ethyl] methanethiosulfonate bromide (MTSET) with cysteines engineered at positions 275, 278, and 282 leads to current inhibition. This effect was state dependent as MTSET appeared less effective at inhibiting IKCa… CONTINUE READING

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