Cysteine‐String Protein

@article{Chamberlain2000CysteineStringP,
  title={Cysteine‐String Protein},
  author={Luke H. Chamberlain and Robert D. Burgoyne},
  journal={Journal of Neurochemistry},
  year={2000},
  volume={74}
}
Abstract: Cysteine‐string protein (Csp) is a major synaptic vesicle and secretory granule protein first discovered in Drosophila and Torpedo. Csps were subsequently identified from Xenopus, Caenorhabditis elegans, and mammalian species. It is clear from the study of a null mutant in Drosophila that Csp is required for viability of the organism and that it has a key role in neurotransmitter release. In addition, other studies have directly implicated Csp in regulated exocytosis in mammalian… 
Cysteine string proteins
The cysteine string protein multimeric complex.
Cysteine string protein‐α is essential for the high calcium sensitivity of exocytosis in a vertebrate synapse
TLDR
The results demonstrate that, in the absence of CSPα, fast Ca2+‐triggered release was not affected at postnatal day (P)14 but was dramatically reduced at P18 and P30 without a change in release kinetics, and suggests that CSP α is indispensable for a normal Ca2- sensitivity of exocytosis in vertebrate mature synapses.
Cysteine String Protein (CSP) Inhibition of N-type Calcium Channels Is Blocked by Mutant Huntingtin*
TLDR
Results indicate that CSP's modulation of G protein inhibition of calcium channel activity is blocked in the presence of a huntingtin fragment with expanded polyglutamine tracts.
Cysteine string protein (CSP) and its role in preventing neurodegeneration
Cysteine string protein expression in mammary epithelial cells
TLDR
The expression of Csp in mammary epithelial cells and in the KIM-2 mammary cell line is demonstrated, and the release of transfected growth hormone (GH) as an assay of secretion is found, suggesting that GH is secreted in an exclusively constitutive manner from Kim-2 cells.
Structure–function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus
TLDR
It is proposed that the cysteine string, in addition to its role in targeting, may be essential for a function of CSP that is dependent on the number of cysteines in the string.
Cysteine string proteins are associated with cortical granules of Xenopus laevis oocytes
TLDR
The presence of csps in fully grown (stage VI) oocytes of the frog, Xenopus laevis, is documented and observations suggest that csps participate in the cortical reaction that underlies the sustained block to polyspermy in Xenopus eggs.
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References

SHOWING 1-10 OF 116 REFERENCES
The Cysteine String Secretory Vesicle Protein Activates Hsc70 ATPase*
TLDR
The results suggest that CSP, a DnaJ family member associated with the secretory vesicle cycle regulates Hsc70 functions, which may function within the biochemical pathways of exo- and endocytosis to promote the formation of multimeric complexes or to regulate conformational changes.
Cysteine string protein functions directly in regulated exocytosis.
TLDR
It is shown that Csp1 overexpression enhances the extent of exocytosis from permeabilized cells in response to Ca2+ or GTPgammaS in the absence of Ca2+.
Mutational analysis of cysteine-string protein function in insulin exocytosis.
TLDR
A functional difference between the two isoforms of Csp wild-type or mutants is demonstrated and a role for the J-domain co-chaperone function as well as for the newly defined linker region in LDCV exocytosis is suggested.
Overexpression of Cysteine-String Proteins inDrosophila Reveals Interactions with Syntaxin
TLDR
It is shown that the individual expression of various CSP isoforms in Drosophila similarly rescues the loss of evoked neurotransmitter release at csp null mutant motor nerve terminals, suggesting widely overlapping functions for each isoform, and that the structural difference of CSP variants may not explain the opposing putative functions of C SP in neurotransmitter and peptide exocytosis.
Cysteine string proteins are associated with cortical granules of Xenopus laevis oocytes
TLDR
The presence of csps in fully grown (stage VI) oocytes of the frog, Xenopus laevis, is documented and observations suggest that csps participate in the cortical reaction that underlies the sustained block to polyspermy in Xenopus eggs.
Identification of a Novel Cysteine String Protein Variant and Expression of Cysteine String Proteins in Non-neuronal Cells (*)
TLDR
It is suggested that Csps may have a more general role in membrane traffic in non-neuronal as well as neuronal cells after the cloning of two Csp variants from bovine adrenal medullary chromaffin cells.
Comparison of Cysteine String Protein (Csp) and Mutant α-SNAP Overexpression Reveals a Role for Csp in Late Steps of Membrane Fusion in Dense-Core Granule Exocytosis in Adrenal Chromaffin Cells
TLDR
Results suggest that unlike α-SNAP, Csp plays a key role in the protein interactions close to the fusion process or fusion pore opening during Ca2+-regulated exocytosis.
The cysteine-string domain of the secretory vesicle cysteine-string protein is required for membrane targeting.
TLDR
It is concluded that although a palmitoylated cysteine-string domain is not required for stable membrane association of Csp, it is essential for initial membrane targeting.
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