Cygnets: in vivo characterization of novel cGMP indicators and in vivo imaging of intracellular cGMP.

@article{Honda2005CygnetsIV,
  title={Cygnets: in vivo characterization of novel cGMP indicators and in vivo imaging of intracellular cGMP.},
  author={Akira Honda and Carolyn L. Sawyer and Sharon M Cawley and Wolfgang R G Dostmann},
  journal={Methods in molecular biology},
  year={2005},
  volume={307},
  pages={27-43}
}
The second messenger cyclic guanosine 5'-monophosphate (cGMP) plays a key role in the control and regulation of a steadily increasing number of diverse physiological processes. As the appreciation of the importance of understanding the cGMP signaling pathway has grown, so has the awareness of the limited techniques with which to study the rapid intracellular cGMP kinetics. We have previously demonstrated the construction of cygnets, cGMP indicators using energy transfer comprised of cyan and… CONTINUE READING

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