Cyclopropenium Nanoparticles and Gene Transfection in Cells

  title={Cyclopropenium Nanoparticles and Gene Transfection in Cells},
  author={Noam Y. Steinman and Luis M. Campos and Yakai Feng and Abraham J. Domb and Hossein Hosseinkhani},
Non-viral vectors for the transfection of genetic material are at the frontier of medical science. In this article, we introduce for the first time, cyclopropenium-containing nanoparticles as a cationic carrier for gene transfection, as an alternative to the common quaternary ammonium transfection agents. Cyclopropenium-based cationic nanoparticles were prepared by crosslinking poly(ethylene imine) (PEI) with tetrachlorocyclopropene. These nanoparticles were electrostatically complexed with… Expand
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  • International Journal of Molecular Sciences
  • 2021
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Quaternary ammonium polysaccharides for gene delivery.
Polycations of dextran grafted with MQ ammonium oligoamines of two to four amino groups were investigated for their ability to cause pCMV-GFP encoding for green fluorescence protein and beta-Gal encoding for beta-galactosidase protein transfection on EPC and HEK-293 cell lines. Expand
Polysaccharide gene transfection agents.
This chapter, compiles the recent progress in polysaccharide based gene delivery, and provides an overview and recent developments of poly Saccharide employed for in vitro and in vivo delivery of therapeutically important nucleotides, e.g. plasmid DNA and small interfering RNA. Expand
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Evaluation of polyplexes as gene transfer agents.
  • C. Gebhart, A. Kabanov
  • Chemistry, Medicine
  • Journal of controlled release : official journal of the Controlled Release Society
  • 2001
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