Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis

@article{Brown1994CyclinlikeAA,
  title={Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis},
  author={Kevin Brown and R M Coulson and T J Yen and Don W Cleveland},
  journal={The Journal of Cell Biology},
  year={1994},
  volume={125},
  pages={1303 - 1312}
}
CENP-E is a kinesin-like protein that binds to kinetochores through the early stages of mitosis, but after initiation of anaphase, it relocalizes to the overlapping microtubules in the midzone, ultimately concentration in the developing midbody. By immunoblotting of cells separated at various positions in the cell cycle using centrifugal elutriation, we… CONTINUE READING