Cyclin is degraded by the ubiquitin pathway

@article{Glotzer1991CyclinID,
  title={Cyclin is degraded by the ubiquitin pathway},
  author={Michael Glotzer and Andrew W. Murray and Marc W. Kirschner},
  journal={Nature},
  year={1991},
  volume={349},
  pages={132-138}
}
Cyclin degradation is the key step governing exit from mitosis and progress into the next cell cycle. When a region in the N terminus of cyclin is fused to a foreign protein, it produces a hybrid protein susceptible to proteolysis at mitosis. During the course of degradation, both cyclin and the hybrid form conjugates with ubiquitin. The kinetic properties of the conjugates indicate that cyclin is degraded by ubiquitin-dependent proteolysis. Thus anaphase may be triggered by the recognition of… Expand
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References

SHOWING 1-10 OF 48 REFERENCES
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity
We show that cyclin plays a pivotal role in the control of mitosis. A proteolysis-resistant mutant of cyclin prevents the inactivation of maturation promoting factor and the exit from mitosis both inExpand
Expression and function of Drosophila cyclin a during embryonic cell cycle progression
TLDR
Immunofluorescent staining revealed that cyclin A accumulates in the interphase cytoplasm of cellularized embryos, but relocates to the nuclear region early in prophase and is completely degraded within metaphase. Expand
How are substrates recognized by the ubiquitin-mediated proteolytic system?
TLDR
A necessary step in the degradation of proteins by the Ubiquitin system is recognition by the ubiquitin-protein ligases(s), with distinct sites on the ligase(s) recognizing specific types of N-termini. Expand
Control of programmed cyclin destruction in a cell-free system
TLDR
A cell-free system from clam embryos that reproduces several aspects of cyclin behavior is developed, and it is shown that cyclin disappearance requires ATP and Mg2+. Expand
Ubiquitin-mediated pathways for intracellular proteolysis.
TLDR
The presence of Ub on histones and on the lymphocyte homing receptor suggests that ubiquitination does not serve exclusively to mark proteins for degradation, and this abundant protein may serve as an intracellular barometer whose distribution among several pools regulates a variety of processes. Expand
Transfer RNA is required for conjugation of ubiquitin to selective substrates of the ubiquitin- and ATP-dependent proteolytic system.
TLDR
It is demonstrated that tRNA is required for conjugation of Ubiquitin to some but not all proteolytic substrates of the ubiquitin mediated pathway, indicating that there are at least two distinct ubiquit in conjugations systems. Expand
The role of cyclin B in meiosis I
TLDR
It is proposed that the unmasking of maternal Cyclin B protein allows it to interact with cdc2 protein kinase, which is also stored in oocytes, and that the formation of this cyclin B/cdc2 complex generates active M phase-promoting factor. Expand
The A‐ and B‐type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.
TLDR
The isolation of a cyclin A cDNA clone from Xenopus eggs shows characteristic accumulation and destruction during mitotic cell cycles, and possible models for the different roles of the A‐ and B‐type cyclins in the control of cell division are discussed. Expand
Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B
TLDR
It is reported that human cyclin A messenger RNA and protein levels also vary during the cell cycle, and increase and decrease in advance of cyclin B levels. Expand
Ubiquitin-mediated protein degradation.
  • A. Hershko
  • Medicine, Biology
  • The Journal of biological chemistry
  • 1988
TLDR
The objective of this Minireview is to summarize briefly what the authors have learned and to point out what they yet have to learn about the mode of action of the ubiquitin-mediated protein breakdown system. Expand
...
1
2
3
4
5
...