Cyclic nucleotides regulate the activity of L-type calcium channels in smooth muscle cells from rat portal vein.

@article{Liu1997CyclicNR,
  title={Cyclic nucleotides regulate the activity of L-type calcium channels in smooth muscle cells from rat portal vein.},
  author={Hsiao-Sheng Liu and Zhiling Xiong and Nick Sperelakis},
  journal={Journal of molecular and cellular cardiology},
  year={1997},
  volume={29 5},
  pages={1411-21}
}
In order to advance our previous findings that the macroscopic slow Ca2+ currents of vascular smooth muscle (VSM) cells are regulated by cyclic nucleotides, the effects of cAMP and cGMP on the activity of single slow (L-type) Ca2+ channels were investigated using cell-attached patch clamp (22-25 degrees C). Freshly isolated VSM cells were obtained from adult male rat portal vein. For the single-channel recordings, the pipette was filled with a solution containing 90 mM Ba2+ and 1 microM Bay-K… CONTINUE READING