Cyclic mechanical stress induces extracellular matrix degradation in cultured chondrocytes via gene expression of matrix metalloproteinases and interleukin-1.

@article{Fujisawa1999CyclicMS,
  title={Cyclic mechanical stress induces extracellular matrix degradation in cultured chondrocytes via gene expression of matrix metalloproteinases and interleukin-1.},
  author={Takuo Fujisawa and Takako Hattori and Kazuhide Takahashi and Takuo Kuboki and Akio Yamashita and Masaharu Takigawa},
  journal={Journal of biochemistry},
  year={1999},
  volume={125 5},
  pages={966-75}
}
To clarify the mechanism of cartilage degradation induced by mechanical stress, we investigated the influence of cyclic tension force (CTF) on the metabolism of cultured chondrocytes. The chondrocytes were exposed to CTF using a Flexercell strain unit. Five or 15 kPa of high frequency CTF significantly inhibited the syntheses of DNA, proteoglycan, collagen, and protein. Fifteen kPa of high frequency CTF induced the expression of interleukin-1 (IL-1), matrix metalloproteinase (MMP)-2 and -9 mRNA… CONTINUE READING