Cyclic adenosine 3',5'-monophosphate (cAMP)-dependent and cAMP-independent regulation of parathyroid hormone receptors on UMR 106-01 osteoblastic osteosarcoma cells.

@article{AbouSamra1991CyclicA3,
  title={Cyclic adenosine 3',5'-monophosphate (cAMP)-dependent and cAMP-independent regulation of parathyroid hormone receptors on UMR 106-01 osteoblastic osteosarcoma cells.},
  author={Abdul Badi Abou-Samra and Jeffrey D. Zajac and D J Schiffer-Alberts and R M Skurat and Anne Kearns and Gino V. Segre and F. Richard Bringhurst},
  journal={Endocrinology},
  year={1991},
  volume={129 5},
  pages={2547-54}
}
The osteoblast-like cells, UMR 106-01, express PTH receptors that are coupled to adenylate cyclase. Recently, we reported the isolation of a UMR 106-01 subclone, UMR 4-7, that is stably transfected with a Zn(++)-inducible mutant of the regulatory subunit of protein kinase A. Incubation of UMR 4-7 cells with Zn++ renders the cells unresponsive to cAMP agonists. This subclone, therefore, seemed particularly suitable for studies of PTH receptor regulation. In UMR 106-01 cells, PTH receptors are… CONTINUE READING