Cyclic AMP induces apolipoprotein E binding activity and promotes cholesterol efflux from a macrophage cell line to apolipoprotein acceptors.

@article{Smith1996CyclicAI,
  title={Cyclic AMP induces apolipoprotein E binding activity and promotes cholesterol efflux from a macrophage cell line to apolipoprotein acceptors.},
  author={Jonathan D Smith and Muneaki Miyata and Mark Ginsberg and C Grigaux and E Shmookler and Andrew S. Plump},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 48},
  pages={30647-55}
}
RAW 264 mouse macrophage cells were stably transfected with human apolipoprotein E (apoE) expression vectors. Clonal derivatives were characterized for expression of the human apoE2, apoE3, and apoE4 isoforms. An apoE4-expressing clonal cell line and a non-expressing clonal control cell line were loaded overnight with either [3H]cholesterol or [3H]choline. The cells were washed and incubated for 24 h in serum-free medium with or without the addition of 8-bromo-cyclic AMP (8-Br-cAMP). Only the… CONTINUE READING
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