Cyanocysteine-mediated molecular dissection of dihydrofolate reductase: occurrence of intra- and inter-molecular reactions forming a peptide bond.

@article{Takenawa1998CyanocysteinemediatedMD,
  title={Cyanocysteine-mediated molecular dissection of dihydrofolate reductase: occurrence of intra- and inter-molecular reactions forming a peptide bond.},
  author={T Takenawa and Yasunori Oda and Yasushi Ishihama and Masahiro Iwakura},
  journal={Journal of biochemistry},
  year={1998},
  volume={123 6},
  pages={1137-44}
}
During a cyanocysteine-mediated dissection study of dihydrofolate reductase, a peptide fragment with a molecular mass of 18 Da less than expected was found as a major reaction product when the dissection reaction was applied to a Lys-cyanocysteine linkage. Detailed characterization of the dissection products by protease digestion, peptide sequencing, liquid chromatography/electrospray ionization mass spectrometry, and capillary electrophoresis suggested that the by-product was generated via a… CONTINUE READING

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