Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters.

  title={Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters.},
  author={Ratnarkar B. Mujumdar and Lauren Ernst and Swati R. Mujumdar and Cara J. Lewis and Alan S. Waggoner},
  journal={Bioconjugate chemistry},
  volume={4 2},
A series of new fluorescent labeling reagents based on sulfoindocyanine dyes has been developed. We describe the synthesis and properties of these reagents. They contain succinimidyl ester reactive groups and can be readily conjugated to antibodies, avidin, DNA, lipids, polymers, and other amino-group-containing materials. The labeling reagents are water soluble, pH insensitive, and show much reduced dye aggregation under labeling conditions. One of the reagents, Cy3, can be excited with the… CONTINUE READING
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