Cutting edge: double-stranded DNA breaks in the IgV region gene were detected at lower frequency in affinity-maturation impeded GANP-/- mice.

@article{Kawatani2005CuttingED,
  title={Cutting edge: double-stranded DNA breaks in the IgV region gene were detected at lower frequency in affinity-maturation impeded GANP-/- mice.},
  author={Yousuke Kawatani and Hideya Igarashi and Takeshi K. Matsui and Kazuhiko Kuwahara and Satoru Fujimura and Nobukazu Okamoto and Katsumasa Takagi and Nobuo Sakaguchi},
  journal={Journal of immunology},
  year={2005},
  volume={175 9},
  pages={5615-8}
}
Double-stranded DNA breaks (DSBs) at the IgV region (IgV) genes might be involved in somatic hypermutation and affinity-maturation of the B cell receptor in response to T cell-dependent Ag. By ligation-mediated PCR, we studied IgV DSBs that occurred in mature germinal center B cells in response to nitrophenyl-chicken gamma-globulin in a RAG1-independent, Ag-dependent, and IgV-selective manner. We quantified their levels in GANP-deficient B cells that have impaired generation of high-affinity Ab… CONTINUE READING