Current two‐dimensional electrophoresis technology for proteomics

  title={Current two‐dimensional electrophoresis technology for proteomics},
  author={Angelika G{\"o}rg and Walter Weiss and Michael J. Dunn},
Two‐dimensional gel electrophoresis (2‐DE) with immobilized pH gradients (IPGs) combined with protein identification by mass spectrometry (MS) is currently the workhorse for proteomics. In spite of promising alternative or complementary technologies (e.g. multidimensional protein identification technology, stable isotope labelling, protein or antibody arrays) that have emerged recently, 2‐DE is currently the only technique that can be routinely applied for parallel quantitative expression… 

Two-dimensional electrophoresis for plant proteomics.

A comprehensive protocol of the current 2-DE technology with IPGs for plant proteome analysis is provided and the individual steps of this technique are described in detail.

Gel-based and gel-free proteomic technologies.

Detailed protocols for global proteomic analysis from adipose-derived stem cells (ASCs) using two central strategies, 2D-DIGE-MS and 2d-LC-MS, are presented here.

Gel‐free mass spectrometry‐based high throughput proteomics: Tools for studying biological response of proteins and proteomes

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Peptide separation with immobilized pI strips is an attractive alternative to in‐gel protein digestion for proteome analysis

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Highlights on the capacities of "Gel-based" proteomics

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Two-dimensional gel electrophoresis in platelet proteomics research.

This chapter presents a protocol for an efficient sample preparation, protein separation by 2-DE, and protein digestion ahead of the MS analysis.

2D gel electrophoresis and mass spectrometry identification and analysis of proteins.

Methods for the analysis of whole cell lysates from human cancer cell lines using 2D-DIGE and identification of differentially expressed proteins using liquid chromatography mass spectrometry, i.e. LC-MS/MS are introduced.

Multidimensional liquid chromatographic separations for proteomics

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Two-dimensional electrophoresis of membrane proteins using immobilized pH gradients.

Improvements have been made in the area of protein solubilization and sample fractionation, leading to a revamp of traditional approaches for 2-DE of membrane proteins.

Preparative two‐dimensional gel electrophoresis of membrane proteins

The modification of a protocol using a combination of 3‐[(3‐cholamidopropyl)‐dimethylammonio]‐1‐propane sulfonate (CHAPS), chaotropic agents (thiourea, urea), Tris base and reducing agents (1,4‐dithioerythritol) to improve solubilization of integral and peripheral membrane proteins.

Advances in protein solubilisation for two‐dimensional electrophoresis

Two‐dimensional (2‐D) electrophoresis remains the highest resolution technique for protein separation and is the method of choice when complex samples need to be arrayed for characterisation, as in

Zooming‐in on the proteome: Very narrow‐range immobilised pH gradients reveal more protein species and isoforms

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The methodology for IPG‐based 2‐DE, since the introduction of the technique in the 1980s, is reviewed and it is shown that in its present form the IPG methodology is mostly useful as a research tool.

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