Current two‐dimensional electrophoresis technology for proteomics
@article{Grg2004CurrentTE, title={Current two‐dimensional electrophoresis technology for proteomics}, author={A. G{\"o}rg and W. Weiss and M. Dunn}, journal={PROTEOMICS}, year={2004}, volume={4} }
Two‐dimensional gel electrophoresis (2‐DE) with immobilized pH gradients (IPGs) combined with protein identification by mass spectrometry (MS) is currently the workhorse for proteomics. In spite of promising alternative or complementary technologies (e.g. multidimensional protein identification technology, stable isotope labelling, protein or antibody arrays) that have emerged recently, 2‐DE is currently the only technique that can be routinely applied for parallel quantitative expression… CONTINUE READING
Topics from this paper
1,757 Citations
Two-dimensional electrophoresis for plant proteomics.
- Chemistry, Medicine
- Methods in molecular biology
- 2007
- 34
High-resolution two-dimensional electrophoresis.
- Chemistry, Medicine
- Methods in molecular biology
- 2009
- 100
Peptide separation with immobilized pI strips is an attractive alternative to in‐gel protein digestion for proteome analysis
- Chemistry, Medicine
- Proteomics
- 2008
- 162
Gel‐free mass spectrometry‐based high throughput proteomics: Tools for studying biological response of proteins and proteomes
- Chemistry, Medicine
- Proteomics
- 2006
- 132
Two-dimensional gel electrophoresis in platelet proteomics research.
- Medicine
- Methods in molecular medicine
- 2007
- 28
2D gel electrophoresis and mass spectrometry identification and analysis of proteins.
- Chemistry, Medicine
- Methods in molecular biology
- 2011
- 15
References
SHOWING 1-10 OF 166 REFERENCES
Two-dimensional electrophoresis of membrane proteins using immobilized pH gradients.
- Chemistry, Medicine
- Analytical biochemistry
- 2000
- 301
Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology.
- Biology, Medicine
- Proceedings of the National Academy of Sciences of the United States of America
- 2000
- 1,291
- PDF
Preparative two‐dimensional gel electrophoresis of membrane proteins
- Chemistry, Medicine
- Electrophoresis
- 1997
- 140
Advances in protein solubilisation for two‐dimensional electrophoresis
- Chemistry, Medicine
- Electrophoresis
- 1999
- 245
Two-dimensional gel electrophoresis: recent advances in sample preparation, detection and quantitation.
- Biology, Medicine
- Current opinion in chemical biology
- 2002
- 244
- PDF
Zooming‐in on the proteome: Very narrow‐range immobilised pH gradients reveal more protein species and isoforms
- Biology, Medicine
- Electrophoresis
- 2001
- 153
An evaluation of the use of two-dimensional gel electrophoresis in proteomics.
- Biology, Medicine
- Biomolecular engineering
- 2001
- 141
Multi‐component immunoaffinity subtraction chromatography: An innovative step towards a comprehensive survey of the human plasma proteome
- Biology, Medicine
- Proteomics
- 2003
- 330
- PDF
Biomedical applications of two‐dimensional electrophoresis using immobilized pH gradients: Current status
- Chemistry, Medicine
- Electrophoresis
- 2000
- 146