Current two‐dimensional electrophoresis technology for proteomics

  title={Current two‐dimensional electrophoresis technology for proteomics},
  author={A. G{\"o}rg and W. Weiss and M. Dunn},
  • A. Görg, W. Weiss, M. Dunn
  • Published 2004
  • Chemistry, Medicine
  • Two‐dimensional gel electrophoresis (2‐DE) with immobilized pH gradients (IPGs) combined with protein identification by mass spectrometry (MS) is currently the workhorse for proteomics. In spite of promising alternative or complementary technologies (e.g. multidimensional protein identification technology, stable isotope labelling, protein or antibody arrays) that have emerged recently, 2‐DE is currently the only technique that can be routinely applied for parallel quantitative expression… CONTINUE READING
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