Current triton X-100 treatments do not allow a complete plasminogen activator extraction from developing nervous tissue

Abstract

Determinations of plaminogen activator (PA) activity are usually performed in Triton X-100-treated tissue homogenates or crude membrane fractions. Such preparations usually involve a single Triton X-100 treatment. In the present paper we describe the pattern of variability of PA activity measured in different fractions obtained from the developing chick CNS by a repetitive procedure of Triton X-100 treatment and ultracentrifugation. To further characterize this PA activity we have also performed zymographic analyses during the embryonic development and the early postnatal life. Our results show that: a) a single Triton X-100 treatment does not completely extract the enzyme and this lead to an underestimation of the total PA activity; b) the PA activity is associated with the particulate component of the total tissue homogenate requiring its complete solubilization more drastic Triton X-100 treatments; c) better estimations of total and specific activities are obtained by using soluble fractions derived by ultracentrifugation from Triton X-100-treated membrane fractions; d) the developing chick optic lobe expresses only one kind of PA molecule along the entire development; e) the level of PA activity vary characteristically during the ontogeny and the early postnatal life indicating the existence of a developmentally regulated mechanism of PA expression.

DOI: 10.1007/BF00970537

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@article{PereyraAlfonso1995CurrentTX, title={Current triton X-100 treatments do not allow a complete plasminogen activator extraction from developing nervous tissue}, author={Susana Pereyra-Alfonso and Gabriel Scicolone and Sara Fiszer de Plazas and Jorge Pecci Saavedra and Vladimir Flores}, journal={Neurochemical Research}, year={1995}, volume={20}, pages={137-142} }