Current trends and challenges in sample preparation for global metabolomics using liquid chromatography-mass spectrometry.

@article{Vuckovic2012CurrentTA,
  title={Current trends and challenges in sample preparation for global metabolomics using liquid chromatography-mass spectrometry.},
  author={Dajana Vuckovic},
  journal={Analytical and bioanalytical chemistry},
  year={2012},
  volume={403 6},
  pages={1523-48}
}
The choice of sample-preparation method is extremely important in metabolomic studies because it affects both the observed metabolite content and biological interpretation of the data. An ideal sample-preparation method for global metabolomics should (i) be as non-selective as possible to ensure adequate depth of metabolite coverage; (ii) be simple and fast to prevent metabolite loss and/or degradation during the preparation procedure and enable high-throughput; (iii) be reproducible; and (iv… CONTINUE READING