Current status and future prospects of using advanced computer-based methods to study bacterial colonial morphology

@article{Bae2016CurrentSA,
  title={Current status and future prospects of using advanced computer-based methods to study bacterial colonial morphology},
  author={Euiwon Bae and Huisung Kim and Bartek Rajwa and John G. Thomas and J. Paul Robinson},
  journal={Expert Review of Anti-infective Therapy},
  year={2016},
  volume={14},
  pages={207 - 218}
}
ABSTRACT Despite the advancement of recent molecular technologies, culturing is still considered the gold standard for microbial sample analysis. Here we review three different bacterial colony–based screening modalities that provide significant information beyond the simple shape and color of the colony. The plate imaging technique provides numeration and quantitative spectral reflectance information for each colony, while Raman spectroscopic analysis of bacteria colonies relates the Raman… Expand
2 Citations
Image analysis of bacterial colonies in classic and alternative gel-based growth media
TLDR
The capacity of flatbed scanners to capture images for the detection and measurement of color development in colonies is assessed and time to the earliest detection of colonies is evaluated, along with colony recovery evaluation, as a means to assess stress induction related to the presence of potentially toxic matrices. Expand
Identification of colonies of cultured shellfish-associated Arcobacter species by Elastic Light Scatter analysis
TLDR
Although substantive differences in the growth rates of some taxa were seen, ELSA was able to differentiate all the species studied, apart from some strains of A. butzleri and A. cryaerophilus, which were nonetheless distinguished from all other species examined. Expand

References

SHOWING 1-10 OF 101 REFERENCES
A novel method for high-throughput data collection in predictive microbiology: optical density monitoring of colony growth as a function of time.
TLDR
A novel optical density method presented to study colony growth based on optical density measurements performed in microtiter plates proved to be a flexible, high-throughput tool for monitoring microbial colony growth in solid(like) systems. Expand
Label‐free identification of bacterial microcolonies via elastic scattering
TLDR
While the proposed method illustrated the possibility of discriminating microcolonies in their early growth stage, more thorough biophysical understanding is needed to expand the technology to other species. Expand
Improvements on colony morphology identification towards bacterial profiling.
TLDR
Since colony morphotyping showed to be a reliable method to evaluate phenotypic switching and also to infer about bacterial diversity in biofilms, these unambiguous comparisons between morphotypes may offer a quite valuable input to clinical diagnosis, aiding the decision-making towards the selection of the most suitable antibiotic and supportive treatments. Expand
Optical forward-scattering for identification of bacteria within microcolonies
TLDR
The discrimination of two species of coagulase-negative Staphylococci on a commercial selective pre-poured medium used in clinical diagnosis without opening lids during the scatterogram acquisition shows the potential of this method—non-invasive, preventing cross-contaminations and requiring minimal dish handling—to provide early clinically-relevant information in the context of fully automated microbiology labs. Expand
Life, Death, and In-Between: Meanings and Methods in Microbiology
  • H. Davey
  • Medicine, Biology
  • Applied and Environmental Microbiology
  • 2011
TLDR
The meaning of microbial viability is explored and guidance is offered in the selection and interpretation of viability testing methods in microbiology laboratories worldwide. Expand
Optical forward-scattering for detection of Listeria monocytogenes and other Listeria species.
TLDR
The overall system proved to be simple, non-invasive and virtually reagent-less and has the potential for automated user-friendly application for detection and differentiation of L. monocytogenes and other Listeria species colonies grown on agar plates within 5-10 min analysis time. Expand
Pattern and control in bacterial colony development.
TLDR
Observation of the dynamics of colony growth, the response of colonies to experimental disruptions of normal development, and the effects of obstacles and of other colonies on gene expression patterns indicate that non-linear responses to chemical gradients in the substrate play an important coordinating role in colony development. Expand
On the application of optical forward-scattering to bacterial identification in an automated clinical analysis perspective.
TLDR
The paper describes an OFS system prototype qualified by the possibility to tune both the laser beam diameter and the acquisition camera field of view, and involves an alternative method for building a reference image database for subsequent image analysis. Expand
Feature extraction from light-scatter patterns of Listeria colonies for identification and classification.
TLDR
An application of computer-vision and pattern-recognition techniques to classify scatter patterns formed by Listeria colonies and is able to determine automatically the pathogenicity of bacteria on the basis of colony scatter patterns is demonstrated. Expand
Development of a microbial high-throughput screening instrument based on elastic light scatter patterns.
TLDR
A microbial high-throughput screening system was developed that enabled high-speed combinatorial studies directly on bacterial colonies and an in-house colony-tracking module enabled researchers to easily understand the time-dependent variation of the ELS from identical colony, which enabled further analysis in other biochemical experiments. Expand
...
1
2
3
4
5
...