Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells

@article{Berlec2013CurrentSA,
  title={Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells},
  author={Ale{\vs} Berlec and B. Strukelj},
  journal={Journal of Industrial Microbiology & Biotechnology},
  year={2013},
  volume={40},
  pages={257-274}
}
Almost all of the 200 or so approved biopharmaceuticals have been produced in one of three host systems: the bacterium Escherichia coli, yeasts (Saccharomyces cerevisiae, Pichia pastoris) and mammalian cells. We describe the most widely used methods for the expression of recombinant proteins in the cytoplasm or periplasm of E. coli, as well as strategies for secreting the product to the growth medium. Recombinant expression in E. coli influences the cell physiology and triggers a stress… CONTINUE READING
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