Current and emerging techniques for ANCA detection in vasculitis

@article{Csernok2014CurrentAE,
  title={Current and emerging techniques for ANCA detection in vasculitis},
  author={Elena Csernok and Frank Moosig},
  journal={Nature Reviews Rheumatology},
  year={2014},
  volume={10},
  pages={494-501}
}
Detection of antineutrophil cytoplasmic antibodies (ANCAs) is a well-established diagnostic test used to evaluate suspected necrotizing vasculitis of small blood vessels. Conditions associated with these antibodies, collectively referred to as ANCA-associated vasculitides, include granulomatosis with polyangiitis (formerly known as Wegener granulomatosis), microscopic polyangiitis, and eosinophilic granulomatosis with polyangiitis (formerly known as Churg–Strauss syndrome). The diagnostic… 

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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
The authors' comparison of various ANCA detection methods showed large variability between the two IIF methods tested and a high diagnostic performance of PR3-ANCA and MPO-AN CA by immunoassay to discriminate AAV from disease controls, indicating that the current international consensus on ANCA testing for AAV needs revision.

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TLDR
Indirect immunofluorescence (IIF) is an excellent technique for screening the presence of ANCAs while other methods such as anti-PR3 and anti- MPO by ELISA or microparticles assays are required for confirmation.

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TLDR
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...

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TLDR
Any report of positive neutrophil fluorescence issued before the ELISA results are available should indicate that positive fluorescence alone is not specific for the diagnosis of Wegener granulomatosis or microscopic polyangiitis and that decisions about treatment should not be based solely on the ANCA results.

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TLDR
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TLDR
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TLDR
A positive result on an enzyme-linked immunosorbent assay ANCA test is not a definitive diagnostic indicator of AAV, but compliance with guidelines for ANCA testing would decrease the number of false-positive results and has the potential to reduce total test expenditures.

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TLDR
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TLDR
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TLDR
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