Curing of endogenous 2 micron DNA in yeast by recombinant vectors

@article{Harford1987CuringOE,
  title={Curing of endogenous 2 micron DNA in yeast by recombinant vectors},
  author={M. N. Harford and Marc Peeters},
  journal={Current Genetics},
  year={1987},
  volume={11},
  pages={315-319}
}
Passaging on selective media of yeast strains transformed with complete 2 micron vectors carrying TRP1, LEU2 or URA3 selective markers leads to curing of the resident endogenous 2 micron DNA in a majority of the population. Vector plasmids defective in FLP function are fixed as populations of A, A + B or B forms after 2 micron loss. Transformation with… CONTINUE READING