Curdlan production by Agrobacterium sp. ATCC 31749 on an ethanol fermentation coproduct

  title={Curdlan production by Agrobacterium sp. ATCC 31749 on an ethanol fermentation coproduct},
  author={Thomas P. West and Beth Nemmers},
  journal={Journal of Basic Microbiology},
The production of the polysaccharide curdlan from the ethanol processing coproduct condensed corn distillers solubles by the bacterium Agrobacterium sp. ATCC 31749 was investigated. It was found that curdlan could be produced by the bacterium using condensed corn distillers solubles as a source of carbon and nitrogen. As the concentration of condensed corn distillers solubles was increased from 50 g l–1 to 400 g l–1, the concentration of curdlan increased but not proportionally. The highest… 
Elevated curdlan production by a mutant of Agrobacterium sp. ATCC 31749
  • T. West
  • Engineering
    Journal of basic microbiology
  • 2009
A mutant strain of the curdlan‐producing bacterium Agrobacterium sp. ATCC 31749, isolated by ethylmethane sulfonate mutagenesis and resistance to ampicillin, was capable of elevated curdlan
Production of the polysaccharide curdlan by an Agrobacterium strain grown on a plant biomass hydrolysate.
The curdlan yield from the cordgrass hydrolysate indicated that the grass was an effective plant biomass substrate for polysaccharide production, independent of the ammonium phosphate concentration present.
Production of the Polysaccharide Curdlan by Agrobacterium species on Processing Coproducts and Plant Lignocellulosic Hydrolysates
This review examines the production of the biopolymer curdlan, synthesized by Agrobacterium species (sp.), on processing coproducts and plant lignocellulosic hydrolysates. Curdlan is a
Effects of carbon sources on production and properties of curdlan using Agrobaterium sp. DH-2
Investigation of the effect of six sugars including glucose, fructose, lactose, maltose, sucrose and xylose as carbon sources on production and properties of curdlan using Agrobacterium sp.
Effect of nitrogen source concentration on curdlan production by Agrobacterium sp. ATCC 31749 grown on prairie cordgrass hydrolysates
  • T. West
  • Chemistry, Medicine
    Preparative biochemistry & biotechnology
  • 2016
ABSTRACT The effect of nitrogen source concentration on the production of the polysaccharide curdlan by the bacterium Agrobacterium sp. ATCC 31749 from hydrolysates of prairie cordgrass was examined.
Metabolic engineering of Agrobacterium sp. ATCC31749 for curdlan production from cellobiose
Three strong promoters were identified and were used to install an energy-efficient cellobiose phosphorolysis mechanism in a curdlan-producing strain, marking the first such metabolic engineering effort in this microbe.
Xanthan gum production from acid hydrolyzed broomcorn stem as a sole carbon source by Xanthomonas campestris
Thermogrvimetric analysis showed that thermal stability of synthesized xanthan gum was similar to those reported in previous studies, and kinetic parameters of the model were determined by fermentation results and evaluated.
Effect of compositional variability of distillers' grains on cellulosic ethanol production.
A review presenting production, characterization, and applications of biopolymer curdlan in food and pharmaceutical sectors
Curdlan was authorized to be used in the food industry by the United States Food and Drug Administration in 1996 and also in 1989 in Taiwan, Japan, and Korea and many patents using curdlan have also been filed from different parts of the world.


Pyrimidine base supplementation effects curdlan production in Agrobacterium sp. ATCC 31749
  • T. West
  • Biology
    Journal of basic microbiology
  • 2006
Pyrimidine base supplementation of the culture medium was found to increase curdlan production by Agrobacterium sp. ATCC 31749. With sucrose as a carbon source, adding uracil or cytosine to the
Production of curdlan-type polysaccharide by Alcaligenes faecalis in batch and continuous culture.
The biosynthesis of a thermogelable, extracellular homo-beta-(1 leads to 3)-glucan called "curdlan," has been studied in batch and continuous cultures of Alcaligenes faecalis var. myxogenes. Curdlan
Theoretical maximum and observed product yields associated with curdlan production by Alcaligenes faecalis.
As a guide to both strain and process improvement, Alcaligenes faecalis var.
Production of β-1,3-glucan exopolysaccharide in low shear systems
A patent culture ofAlcaligenes faecalis var.myxogenes (ATCC 31749) synthesizes “curdlan” during the stationary phase of an aerobic batch fermentation following the depletion of assimilable nitrogen.
Growth and β-Glucan 10C3K Production by a Mutant of Alcaligenes faecalis var. myxogenes in Defined Medium
A defined medium was developed in which Alcaligenes faecalis var. myxogenes 10C3 mutant K produced a large quantity of β-glucan 10C3K. The medium contained 4% glucose together with 0.1% citrate,
Cloning and Characterization of the Phosphatidylserine Synthase Gene of Agrobacterium sp. Strain ATCC 31749 and Effect of Its Inactivation on Production of High-Molecular-Mass (1→3)-β-d-Glucan (Curdlan)
One mutant was unable to produce high-molecular-mass curdlan when a previously uncharacterized gene, pss(AG), encoding a 30-kDa, membrane-associated phosphatidylserine synthase was disrupted, and the continued appearance of PC revealed that its production by this Agrobacterium strain is not solely dependent on PE.
The future of coproducts from corn processing
  • K. Rausch, R. Belyea
  • Environmental Science, Medicine
    Applied biochemistry and biotechnology
  • 2006
The development of more efficient methods of water removal would increase the efficiency of ethanol processing and reduce the costs of processing, and new technologies could contribute to greater stability of dry-grind plants.
Higher intracellular levels of uridinemonophosphate under nitrogen-limited conditions enhance metabolic flux of curdlan synthesis in Agrobacterium species.
It is concluded that only the higher intracellular UMP levels caused by nitrogen limitation in the medium enhance the metabolic flux of curdlan synthesis by promoting cellular UDP-glucose synthesis.
Curdlan: Properties and Application to Foods
Gel formation of curdlan at 100–130°C and behaviors of curdlan gels under frozen state were investigated by measuring gel strength and syneresis. Even when aqueous suspensions of curdlan were heated