Culturing the epiblast cells of the pig blastocyst

  title={Culturing the epiblast cells of the pig blastocyst},
  author={Neil C Talbot and Caird E. Rexroad and Vernon G. Pursel and Anne M. Powell and Neil D. Nel},
  journal={In Vitro Cellular & Developmental Biology - Animal},
Pig epiblast cells that had been separated from other early embryonic cells were cultured in vitro. A three-step dissection protocol was used to isolate the epiblast from trophectoderm and primitive endoderm before culturing. Blastocysts collected at 7 to 8 days postestrus were immunodissected to obtain the inner cell mass (ICM) and destroy trophectodermal cells. The ICM was cultured for 2 to 3 days on STO feeder cells. The epiblast was then physically dissected free of associated primitive… CONTINUE READING

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