Culturing and direct DNA extraction find different fungi from the same ericoid mycorrhizal roots.

@article{Allen2003CulturingAD,
  title={Culturing and direct DNA extraction find different fungi from the same ericoid mycorrhizal roots.},
  author={T. R. Allen and Tony Millar and Shannon M. Berch and Mary L. Berbee},
  journal={The New phytologist},
  year={2003},
  volume={160 1},
  pages={
          255-272
        }
}
•  This study compares DNA and culture-based detection of fungi from 15 ericoid mycorrhizal roots of salal (Gaultheria shallon), from Vancouver Island, BC Canada. •  From the 15 roots, we PCR amplified fungal DNAs and analyzed 156 clones that included the internal transcribed spacer two (ITS2). From 150 different subsections of the same roots, we cultured fungi and analyzed their ITS2 DNAs by RFLP patterns or sequencing. We mapped the original position of each root section and recorded fungi… 
View on PubMed
schirp.sites.olt.ubc.ca
200 Citations
Highly Influential Citations
29
Background Citations
102
Methods Citations
19
Results Citations
12

Figures from this paper

200 Citations

Assemblages of ericoid mycorrhizal and other root-associated fungi from Epacris pulchella (Ericaceae) as determined by culturing and direct DNA extraction from roots.

The most abundant RFLP types in the cultured isolate assemblage were identified as putative ericoid mycorrhizal ascomycete endophytes, and these also represented the most abundant rDNA internal transcribed spacer (ITS) restriction fragment length polymorphisms (RFLPs) in the cloned assemblages and the most intense bands in DGGE profiles.

Fungi associated with hair roots of Rhododendron lochiae (Ericaceae) in an Australian tropical cloud forest revealed by culturing and culture-independent molecular methods.

A combination of culturing and culture-independent approaches may be more efficacious than either method individually, and several unique fungi are identified.

Identical genotypes of an ericoid mycorrhiza-forming fungus occur in roots of Epacris pulchella (Ericaceae) and Leptospermum polygalifolium (Myrtaceae) in an Australian sclerophyll forest.

Assemblages of fungi associated with roots of cooccurring Epacris pulchella and Leptospermum polygalifolium seedlings at a sclerophyll forest site in New South Wales, Australia, were investigated and distribution of genotypes of an ERM Helotiales ascomycete in root systems of the two plant taxa was investigated using inter-simple sequence repeat-PCR.

PCR Primers with Enhanced Specificity for Nematode-Trapping Fungi (Orbiliales)

Orbiliales-specific PCR primers for the ITS and 28s rDNA were developed to directly detect nematode-trapping fungi without culturing and also to screen fungal isolates for phylogenetic placement in the Orbiliales.

Diversity of root-associated fungal endophytes in Rhododendron fortunei in subtropical forests of China

The endophyte population isolated from R. fortunei is composed mainly of ascomycete, as well as a few basidiomycete strains, which appears to have strong affinities for some unidentified root endophytes from Ericaceae hosts in Australian forests.

Diversity and characteristics of colonization of root-associated fungi of Vaccinium uliginosum

It is suggested that under certain environmental stresses, such as low temperature and poor available nutrients, ericoid plants may favor co-colonization by both EMF and DSE, which is similar to the results of the percent colonization of DSE by the magnified intersections method.

Diverse Helotiales associated with the roots of three species of Arctic Ericaceae provide no evidence for host specificity.

Data suggest that ericaceous roots host diverseFungal communities dominated by the Helotiales, however, these fungal communities are unlikely to be controlled by fungal host preferences and the mechanisms maintaining high diversity in root-symbiotic communities remain to be elucidated.

Host Associations Between Fungal Root Endophytes and Boreal Trees

The results indicate that fungal root endophytes of boreal trees are not randomly distributed, but instead form relatively distinct assemblages on different host tree species.

Co-existing ericaceous plant species in a subarctic mire community share fungal root endophytes

Diversity of culturable ericoid mycorrhizal fungi of Rhododendron decorum in Yunnan, China

The diversity of ericoid mycorrhizal fungi isolated from Rhododendron decorum Franch in Yunnan, southwestern China, was examined for the first time and these were determined by morphological and molecular means to belong to 12 fungal species.
...

References

SHOWING 1-10 OF 31 REFERENCES

Are Sebacinaceae common and widespread ectomycorrhizal associates of Eucalyptus species in Australian forests?

A molecular survey of basidiomycete ectomycorrhizal fungi colonising root tips at a site in Eucalyptus marginata (jarrah) forest revealed the presence of many fungal species which could not be identified from a database of ITS-PCR-RFLP profiles from morphologically identified species and revealed a close relationship among the three fungi.

Diversity and abundance of ericoid mycorrhizal fungi of Gaultheria shallon on forest clearcuts

The association in the laboratory between A. strictum and salal was atypical in that the fungus improved the growth of salal seedlings but was slow to colonize roots and occasi...

Molecular diversity within and between ericoid endophytes from the Ericaceae and Epacridaceae

Comparing similarities and maximum-parsimony analysis suggests that these isolates from the Epacridaceae do not belong to the same species as those forming ericoid mycorrhiza in the Ericaceae.

ITS primers with enhanced specificity for basidiomycetes ‐ application to the identification of mycorrhizae and rusts

ITS1‐F/ITS4‐B preferential amplification was shown to be particularly useful for detection and analysis of the basidiomycete component in ectomycorrhizae and in rust‐infected tissues.

Ericoid mycorrhizal fungi are common root associates of a Mediterranean ectomycorrhizal plant (Quercus ilex)

The results indicate that ericoid mycorrhizal fungi associate with ectomycorrhiza roots of neighbouring Quercus ilex and Erica arborea plants and the ecological significance of this finding is discussed.

Molecular detection, community structure and phylogeny of ericoid mycorrhizal fungi

It is determined that five putative species and 2 polyphyletic assemblages of fungi produce ericoid mycorrhizae in Gaultheria shallon, other Ericaceae and Epacridaceae, and using phylogenetic analysis of ITS2 sequences in GenBank, it is confirmed that most of these fungi occur in North America, Europe, and Australia.

Ectomycorrhizas involving sebacinoid mycobionts.

Observations on ectomycorrhizas and basidiomes suggest that species of Sebacinaceae are fairly common mycobionts in various ectomyCorrhizal plant communities and the complex trophic relationships in the SebacInaceae are discussed.

Ericoid mycorrhizal fungi: some new perspectives on old acquaintances

Investigation of genetic diversity and molecular ecology of ericoid mycorrhizal fungi and the molecular analysis of sterile morphotypes involved in this symbiosis has revealed that ericaceous plants can be very promiscuous, with multiple occupancy of their thin roots.

The biology of mycorrhiza in the Ericaceae

It was revealed that H. ericae was able to produce significantly higher yield when grown on intact fungal necromass than when provided with equivalent concentrations of N in the form of ammonium.

Basal hymenomycetes belonging to the Sebacinaceae are ectomycorrhizal on temperate deciduous trees.

Two sebacinoids of divergent rDNA sequences were demonstrated to form similar ectomycorrhizas, with a well-developed Hartig net and a hyphal mantle having thick-walled outer mantle hyphae.